Studies on monoclonal anti-isotypic and anti-idiotypic antibodies against leukemia and myeloma: i. study on monoclonal anti-idiotypic antibodies against λ chain protein of myeloma
November 19th, 2008 by 
admin Summary We utilised A concern extracted from piddle of enduring with myeloma (IgD λ) as antigen for immunizing BALB/C mice, and 86 hybridoma
radiophone clones secreting monoclonal antibodies (McAb) were obtained after fusing twice and cloning 3–4 times. 15 of these clones
secreted monoclonal anti-idiotypic antibodies (anti-Id McAb). The results showed that 12 of 15 anti-Id antibodies reacted
exclusive with homological λ concern and IgD, not with A chain, κ chain, IgG, IgA, IgM, IgD, IgE, toxin and paraglobulin from normal
subjects. Indirect immunofluorescent assessment demonstrated that constructive activity evaluate between anti-Id McAbs and marginal blood
lymphocytes or pearl goody cells of the enduring with myeloma was up to 23 %. No activity was observed between anti-Id McAb
and marginal blood…
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